Specificity of Pseudomonas Isoamylase
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چکیده
منابع مشابه
Transcription of the isoamylase gene (iam) in Pseudomonas amyloderamosa SB-15.
S1 nuclease mapping of RNA prepared from Pseudomonas amyloderamosa SB-15 suggested that the iam gene coding for isoamylase (glycogen 6-glucanohydrolase [EC 3.2.1.68]) is transcribed from two promoters. The transcription start site for the upstream promoter (termed P1) was located -182 base pairs from the first nucleotide of the initiation codon of iam, whereas the start site for the downstream ...
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Amylase isoenzyme analysis was made of extracts of normal human pancreatic tissue by first conducting ion exchange chromatography of the purified material. This gave evidence of only pancreatic type (P-type) isoamylase for all purposes. However, when effluent fractions in which salivary type isoamylase would ordinarily be expected to be present were harvested, pooled, concentrated, and rechroma...
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A culture of a coliform bacteria isolated from soil produced isoamylase when grown in peptone-maltose-salts broth. The enzyme preparation was partially purified. Its similarity with isoamylase produced by Aerobacter aerogenes was established by studying its action upon various polysaccharides. A study of the organism producing isoamylase showed that it differed from A. aerogenes in some of its ...
متن کاملGlycosylation substrate specificity of Pseudomonas aeruginosa 1244 pilin.
The beta-carbon of the Pseudomonas aeruginosa 1244 pilin C-terminal Ser is a site of glycosylation. The present study was conducted to determine the pilin structures necessary for glycosylation. It was found that although Thr could be tolerated at the pilin C terminus, the blocking of the Ser carboxyl group with the addition of an Ala prevented glycosylation. Pilin from strain PA103 was not gly...
متن کاملGlycosylation of Pseudomonas aeruginosa 1244 pilin: glycan substrate specificity.
The structural similarity between the pilin glycan and the O-antigen of Pseudomonas aeruginosa 1244 suggested that they have a common metabolic origin. Mutants of this organism lacking functional wbpM or wbpL genes synthesized no O-antigen and produced only non-glycosylated pilin. Complementation with plasmids containing functional wbpM or wbpL genes fully restored the ability to produce both O...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1969
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.33.625